IB47221 Mini Genomic Tissue DNA Kit for 50 Preparations

The Genomic DNA mini kit (tissue) was designed specifically for purifying total DNA (including genomic, mitochondrial and viral DNA) from a variety of animal tissue, paraffin-embedded tissue, buccal swab and amniotic fluid. The provided micropestle can efficiently homogenize tissue samples to shorten the time in the Lysis Step. Proteinase K and chaotropic salt are used to lyse cells and degrade protein, allowing DNA to be easily bound by the glass fiber matrix of the spin column (1). Once any contaminants have been removed, using a Wash Buffer (containing ethanol), the purified DNA is eluted by a low salt Elution Buffer or TE. The entire procedure can be completed within 1 hour without phenol/chloroform extraction or alcohol precipitation. The expected yield of genomic DNA is up to 50 microgram and the purified DNA (with approximately 20-30 Kb) is suitable for use in PCR or other enzymatic reactions. The quality of the Genomic DNA mini kit (tissue) is tested on a lot-to-lot basis by isolating genomic DNA from a 20 mg mouse liver sample. The Purified DNA (more than 10 microgram with an A260/A280 ratio of 1.7 - 1.9) is quantified with a spectrophotometer and checked by electrophoresis. Caution: GBT Buffer contains guanidine hydrochloride which is a harmful irritant. During operation, always wear a lab coat, disposable gloves, and protective goggles. Operation: centrifuge/vacuum manifold. Expectant yield: up to 50 microgram. Binding capacity: 50 microgram. Operation time: 60 minutes or less. Elution volume: 50-200 microliter. Applications: PCR, AFLP/PADP, RFLP, southern blotting, real-time PCR.

• Kit contains 50 Mini GD columns, (100) 2mL collection tubes, (50) micropestle, 30mL GT buffer, 30mL GB buffer, 45mL W1 buffer
• It also contains 25mL wash buffer (100mL add ethanol), 30mL elution buffer, 11mg Proteinase K (1.1mL add ddH2O)
• For research use only, extract genomic DNA from blood, cultured cells, and various animal tissues
• Sample size: up to 50mg of tissue or 200 microliter of blood
• Spin column format